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Differentiation of 3T3-L1 pre-adipocytes 3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work.[18] Briefly, standard culture media was supplemented with scheduled addition of adipogenic agents [3-isobutyl-1methyl xanthine (IBMX), Sigma Aldrich Do You Need a Prescription for B12 Injections? | OmegaQuant Size:1000 mcg/ml - 10 ml x 4 https://lookaside.fbsbx.com/lookaside/crawler/media/?media_id=1229695905186775 Type_Grate & Drainage

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