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Binding assays using [ 3 H]-Compound 21 confirmed that Compound 21 binds to the ghrelin receptor construct, although the binding affinity of the construct (dissociation constant, K d = 17.2 ± 7.1 nM) was lower than that of the WT ghrelin receptor ( K d = 4.42 ± 0.76 nM) (Supplementary Fig