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To construct GST-tagged LIN28A variants, including full-length (1–209), 1–77, 78–163, 164–209, 1–124, and 125–209, the vector, pGEX-4T-1 (GE Healthcare Life Sciences), and PCR-amplified product from pFLAG-LIN28A using primers LIN28A_XhoI-F and LIN28A_NotI-R for 1–209, LIN28A_XhoI-F and LIN28A(1–77)_NotI-R for 1–77, LIN28A(78–163)_XhoI-F and LIN28A(78–163)_NotI-R for 78–163, LIN28A(164–209)_XhoI-F and LIN28A_NotI-R for 164–209, LIN28A_XhoI-F and LIN28A(1–124)_NotI-R for 1–124, and LIN28A(125–209)_XhoI-F and LIN28A_NotI-R for 125–209 were digested with Xho I (NEB, R0146) and Not I (NEB, R0189) and ligated

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Moreover, dual IGF-1R/INSR inhibition restored the efficacy of both chemotherapy drugs and improved in vitro and in vivo survival [24]