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The samples were centrifuged, and the supernatant was subjected to a GSH assay based on a kinetic enzymatic recycling method that detects the oxidation of GSH by 5,5′-dithiobis-2-nitrobenzoic acid (DTNB) and glutathione reductase to measure the GSH content in cells 74

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References Primary preclinical: McCoy, Rush, Yang et al., "Evaluation of metabolically stabilized angiotensin IV analogs as procognitive/antidementia agents," Journal of Pharmacology and Experimental Therapeutics, 2013 (PMID 23055539) — the foundational dihexa paper and the source of the dosing, half-life, brain-to-plasma and dendritic spine figures cited throughout this page