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All lanes: Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/1000 dilution Lane 1: Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg Lane 2: GPX4 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg Lane 2: Western blot - Human GPX4 knockout HeLa cell line (ab262509) Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg Predicted band size: 22 kDa Observed band size: 20 kDa false WB Lab Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066) Blocking/Diluting buffer and concentration 5% NFDM/TBST
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In vitro, a T2 cytokine (IL-13) induced 15LO1 generation of hydroperoxy-phospholipids, which lowered intracellular GSH and increased extracellular GSSG
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