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Flow cytometry Surface staining Total blood cells, or 5 × 10 6 cells from the liver or the spleen, were incubated with anti-CD16/32 (1:200, BD Pharmingen, 553142) and Ghost Dye Violet 540 (1:1000, Tonbo Bioscience, 13-0879-T500) for 15 min at 4 °C
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