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Western blot analysis For the generation of whole cell extracts, αTC1 and αTC1 KO cells, as well as αTC1 cells exposed to CX-4945, SGC-CK2-1 or DMSO as control for 24 h, were harvested and lysed for 30 min at 4°C with lysis buffer (10 mmol/l Tris-HCl, pH 7.5, 10 mmol/l NaCl, 0.1 mmol/l EDTA, 0.5% [vol./vol.] Triton X-100, 0.02% [wt/vol.] NaN 3 ) supplemented with 0.5 mmol/l phenylmethylsulfonyl fluoride (PMSF) and a protease and phosphatase inhibitor cocktail (1:75 vol./vol., Sigma-Aldrich)

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however, an excess of certain oxidants, classified as reactive oxygen species (ROS), can cause cell death and oxidative stress